Simultaneous Estimation of Pioglitazone Hcl and Glimepiride by Rp-hplc Method

The main objective of the present work is to develop a new simple RPHPLC method for simultaneous estimation of Pioglitazone HCl and Glimepiride. A series of mobile phases were tried, among the various mobile phases 0.05 M Disodium hydrogen phosphate Buffer (pH 3± 0.2) Methanol and Acetonitrile in the ratio of 45:30:25 was found to be an ideal mobile phase since it gave a good resolution and peak shapes with perfect optimization. The flow rate was found to be optimized at 1 ml/min.The linearity and range was found to be in the range of 30-150 g/ml for Pioglitazone HCl and for Glimepiride 4-20 g/ml. The correlation coefficient of Pioglitazone HCl and for Glimepiride was found to be 0.9982 and 0.9978 respectively, which indicates a perfect correlation. The developed method was validated for accuracy, precision, and system suitability. The percentage recovery of Pioglitazone HCl and for Glimepiride was found to be 99.77% and 100.06% respectively. The good percentage recovery of the sample clearly indicates the reproducibility and accuracy of the developed method. Similarly the % RSD value for precision was also found to be within the acceptable limit.